Finally, Move-in Day at One Bryant Park!

The trading floor at the new Bank of America tower at One Bryant Park got partially busy on Monday, three days after the building’s developer secured a temporary certificate of occupancy for Manhattan’s newest trophy tower.

Three hundred Bank of America associates from its global corporate and investment arm began relocating to the trading floor of the crystalline horizon-scratcher, which the bank co-owns with developer the Durst Organization. Ultimately, a staff of 4,000 will work there.

Bank of America is consolidating its operations within 1.6 million square feet of space at the 54-story skyscraper, on Sixth Avenue, between 42nd and 43rd streets. Right now, many associates trade from scattered offices in buildings like 9 and 40 West 57th Street.

Four years after construction began, only the 37th floor remains unleased in the new top-tier tower, which, however temporarily, is the city’s second-tallest. Tenants include law firm Akin Gump, Al Gore’s Generation Investment and Durst’s offices.

The building will have an official opening, complete with the hoopla appropriate to the rise of a new Manhattan trophy, sometime next year.

For now, much work still remains to be done (the 51st floor doesn’t even have walls yet).

Even so, John Yiannacopoulos, a spokesman for Bank of America, said the associates were stoked about their new digs.

“It’s a remarkable building,” Mr. Yiannacopoulos said. “Suffice it to say that the associates are really excited to move into a tower that’s environmentally friendly and really the first of its kind in the nation.”